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w v sds b7024s and b7025s new england biolabs  (New England Biolabs)


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    New England Biolabs w v sds b7024s and b7025s new england biolabs
    W V Sds B7024s And B7025s New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/purple+6x/Gel+Loading+Dye%2C+Purple+(6X)%2C+no+SDS/pmc13132344-103-68-73
    Average 97 stars, based on 480 article reviews
    w v sds b7024s and b7025s new england biolabs - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Hyperspectral differentiation of three grapevine yellows diseases and symptomatically similar stresses
    Article Snippet: The gel was stained with Serva DNA Stain Clear G (SERVA Electrophoresis GmbH, Heidelberg, Germany). .. Ten μL of PCR products were stained with 1.5 μL Gel Loading Dye, Purple (6X) (New England Biolabs GmbH, Frankfurt am Main, Germany) and loaded on the gel. .. Quantitas Pro DNA Marker 50 bp – 1.5 kb (Biozym Scientific GmbH, Hessisch Oldendorf, Germany) were loaded on the gel to confirm the size of the amplified fragment of the virus.

    Article Title: Threonine Aldolase-Catalyzed Stereoselective Radical α -Alkylation of Amino Acids
    Article Snippet: 100 ng/μL as determined by NanoDrop); prepared by long-range PCR using pET-28a(+) as the template and 007 and 008 as primers. .. Phusion ® High-Fidelity DNA Polymerase, DMSO, Phusion ® 5X HF Buffer, dNTPs, DpnI, from New England Biolabs (NEB) PCR water (autoclaved Milli-Q water) 50X TAE buffer (50 mM EDTA, 2 M Tris, 1 M acetic acid) 1% agarose gel (5 g agarose (low electroendosmosis, GOLDBIO, Catalog number: A–201–500) in 500 mL 1X TAE buffer, microwave until transparent) 1 kb DNA ladder (NEB, Catalog number: N3232S) Gel loading dye, purple (6X), no SDS (NEB, Catalog number: B7025S) with SYBR ® Gold nucleic acid gel stain 10,000X concentrate in DMSO (Invitrogen, Catalog number: S11494) Monarch ® PCR & DNA Gel Extraction Kit (NEB) Gibson Mastermix 28 (320 μL 5X isothermal buffer (25% PEG-8000, 500 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 50 mM dithiothreitol, 1 mM each of the dNTPs, and 5 mM NAD)), 0.64 μL of 10 U/μL T5 exonuclease, 20 μL of 2 U/μL Phusion ® High-Fidelity DNA Polymerase, 160 μL of 40 U/μL Taq DNA ligase from NEB) Purified double-stranded DNA fragments with 18–22 bp overlaps SOC medium (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose, autoclaved) Electrocompetent E. coli strain E. cloni BL21(DE3) cells (Lucigen) LB kan agar plate (15 g agar (molecular genetics grade, Fisher BioReagents TM , Catalog number: BP1423–500) in Luria-Bertani medium with 50 μg/mL kanamycin monosulfate salt, Chem-Impex Int’l. ..

    Article Title: An improved model for prediction of de novo designed proteins with diverse geometries
    Article Snippet: .. Each 25 μL PCR reaction contained: 12.5 μL 2x Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs, Product Number: M0494S) 1.25 μL Forward Primer (from 10 μM stock) 1.25 μL Reverse Primer (from 10 μM stock) 2.5 μL DNA template (from 1 ng/μL stock) 7.5 μL water PCR cycling protocol: 30 seconds at 98°C 10 seconds at 98°C 30 seconds at 63°C 42.5 seconds at 72°C 120 seconds at 72°C Hold at 12°C A 120 mL agarose (0.8% weight/volume) gel was prepared by adding: 120 mL Tris-Acetate-EDTA (TAE) buffer 0.96 g agarose 12 μL GelRed Nucleic Acid Gel Stain (Biotium, Product Number: 41003) 2 μL of each of the 39 PCR products were mixed with 1 μL of Gel Loading Dye, Purple (6X) (New England Biolabs, Product Number: B7024S) and 3 μL of water. ..

    Staining:

    Article Title: Hyperspectral differentiation of three grapevine yellows diseases and symptomatically similar stresses
    Article Snippet: The gel was stained with Serva DNA Stain Clear G (SERVA Electrophoresis GmbH, Heidelberg, Germany). .. Ten μL of PCR products were stained with 1.5 μL Gel Loading Dye, Purple (6X) (New England Biolabs GmbH, Frankfurt am Main, Germany) and loaded on the gel. .. Quantitas Pro DNA Marker 50 bp – 1.5 kb (Biozym Scientific GmbH, Hessisch Oldendorf, Germany) were loaded on the gel to confirm the size of the amplified fragment of the virus.

    Article Title: Threonine Aldolase-Catalyzed Stereoselective Radical α -Alkylation of Amino Acids
    Article Snippet: 100 ng/μL as determined by NanoDrop); prepared by long-range PCR using pET-28a(+) as the template and 007 and 008 as primers. .. Phusion ® High-Fidelity DNA Polymerase, DMSO, Phusion ® 5X HF Buffer, dNTPs, DpnI, from New England Biolabs (NEB) PCR water (autoclaved Milli-Q water) 50X TAE buffer (50 mM EDTA, 2 M Tris, 1 M acetic acid) 1% agarose gel (5 g agarose (low electroendosmosis, GOLDBIO, Catalog number: A–201–500) in 500 mL 1X TAE buffer, microwave until transparent) 1 kb DNA ladder (NEB, Catalog number: N3232S) Gel loading dye, purple (6X), no SDS (NEB, Catalog number: B7025S) with SYBR ® Gold nucleic acid gel stain 10,000X concentrate in DMSO (Invitrogen, Catalog number: S11494) Monarch ® PCR & DNA Gel Extraction Kit (NEB) Gibson Mastermix 28 (320 μL 5X isothermal buffer (25% PEG-8000, 500 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 50 mM dithiothreitol, 1 mM each of the dNTPs, and 5 mM NAD)), 0.64 μL of 10 U/μL T5 exonuclease, 20 μL of 2 U/μL Phusion ® High-Fidelity DNA Polymerase, 160 μL of 40 U/μL Taq DNA ligase from NEB) Purified double-stranded DNA fragments with 18–22 bp overlaps SOC medium (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose, autoclaved) Electrocompetent E. coli strain E. cloni BL21(DE3) cells (Lucigen) LB kan agar plate (15 g agar (molecular genetics grade, Fisher BioReagents TM , Catalog number: BP1423–500) in Luria-Bertani medium with 50 μg/mL kanamycin monosulfate salt, Chem-Impex Int’l. ..

    Article Title: An improved model for prediction of de novo designed proteins with diverse geometries
    Article Snippet: .. Each 25 μL PCR reaction contained: 12.5 μL 2x Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs, Product Number: M0494S) 1.25 μL Forward Primer (from 10 μM stock) 1.25 μL Reverse Primer (from 10 μM stock) 2.5 μL DNA template (from 1 ng/μL stock) 7.5 μL water PCR cycling protocol: 30 seconds at 98°C 10 seconds at 98°C 30 seconds at 63°C 42.5 seconds at 72°C 120 seconds at 72°C Hold at 12°C A 120 mL agarose (0.8% weight/volume) gel was prepared by adding: 120 mL Tris-Acetate-EDTA (TAE) buffer 0.96 g agarose 12 μL GelRed Nucleic Acid Gel Stain (Biotium, Product Number: 41003) 2 μL of each of the 39 PCR products were mixed with 1 μL of Gel Loading Dye, Purple (6X) (New England Biolabs, Product Number: B7024S) and 3 μL of water. ..

    Agarose Gel Electrophoresis:

    Article Title: Threonine Aldolase-Catalyzed Stereoselective Radical α -Alkylation of Amino Acids
    Article Snippet: 100 ng/μL as determined by NanoDrop); prepared by long-range PCR using pET-28a(+) as the template and 007 and 008 as primers. .. Phusion ® High-Fidelity DNA Polymerase, DMSO, Phusion ® 5X HF Buffer, dNTPs, DpnI, from New England Biolabs (NEB) PCR water (autoclaved Milli-Q water) 50X TAE buffer (50 mM EDTA, 2 M Tris, 1 M acetic acid) 1% agarose gel (5 g agarose (low electroendosmosis, GOLDBIO, Catalog number: A–201–500) in 500 mL 1X TAE buffer, microwave until transparent) 1 kb DNA ladder (NEB, Catalog number: N3232S) Gel loading dye, purple (6X), no SDS (NEB, Catalog number: B7025S) with SYBR ® Gold nucleic acid gel stain 10,000X concentrate in DMSO (Invitrogen, Catalog number: S11494) Monarch ® PCR & DNA Gel Extraction Kit (NEB) Gibson Mastermix 28 (320 μL 5X isothermal buffer (25% PEG-8000, 500 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 50 mM dithiothreitol, 1 mM each of the dNTPs, and 5 mM NAD)), 0.64 μL of 10 U/μL T5 exonuclease, 20 μL of 2 U/μL Phusion ® High-Fidelity DNA Polymerase, 160 μL of 40 U/μL Taq DNA ligase from NEB) Purified double-stranded DNA fragments with 18–22 bp overlaps SOC medium (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose, autoclaved) Electrocompetent E. coli strain E. cloni BL21(DE3) cells (Lucigen) LB kan agar plate (15 g agar (molecular genetics grade, Fisher BioReagents TM , Catalog number: BP1423–500) in Luria-Bertani medium with 50 μg/mL kanamycin monosulfate salt, Chem-Impex Int’l. ..

    Gel Extraction:

    Article Title: Threonine Aldolase-Catalyzed Stereoselective Radical α -Alkylation of Amino Acids
    Article Snippet: 100 ng/μL as determined by NanoDrop); prepared by long-range PCR using pET-28a(+) as the template and 007 and 008 as primers. .. Phusion ® High-Fidelity DNA Polymerase, DMSO, Phusion ® 5X HF Buffer, dNTPs, DpnI, from New England Biolabs (NEB) PCR water (autoclaved Milli-Q water) 50X TAE buffer (50 mM EDTA, 2 M Tris, 1 M acetic acid) 1% agarose gel (5 g agarose (low electroendosmosis, GOLDBIO, Catalog number: A–201–500) in 500 mL 1X TAE buffer, microwave until transparent) 1 kb DNA ladder (NEB, Catalog number: N3232S) Gel loading dye, purple (6X), no SDS (NEB, Catalog number: B7025S) with SYBR ® Gold nucleic acid gel stain 10,000X concentrate in DMSO (Invitrogen, Catalog number: S11494) Monarch ® PCR & DNA Gel Extraction Kit (NEB) Gibson Mastermix 28 (320 μL 5X isothermal buffer (25% PEG-8000, 500 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 50 mM dithiothreitol, 1 mM each of the dNTPs, and 5 mM NAD)), 0.64 μL of 10 U/μL T5 exonuclease, 20 μL of 2 U/μL Phusion ® High-Fidelity DNA Polymerase, 160 μL of 40 U/μL Taq DNA ligase from NEB) Purified double-stranded DNA fragments with 18–22 bp overlaps SOC medium (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose, autoclaved) Electrocompetent E. coli strain E. cloni BL21(DE3) cells (Lucigen) LB kan agar plate (15 g agar (molecular genetics grade, Fisher BioReagents TM , Catalog number: BP1423–500) in Luria-Bertani medium with 50 μg/mL kanamycin monosulfate salt, Chem-Impex Int’l. ..

    Purification:

    Article Title: Threonine Aldolase-Catalyzed Stereoselective Radical α -Alkylation of Amino Acids
    Article Snippet: 100 ng/μL as determined by NanoDrop); prepared by long-range PCR using pET-28a(+) as the template and 007 and 008 as primers. .. Phusion ® High-Fidelity DNA Polymerase, DMSO, Phusion ® 5X HF Buffer, dNTPs, DpnI, from New England Biolabs (NEB) PCR water (autoclaved Milli-Q water) 50X TAE buffer (50 mM EDTA, 2 M Tris, 1 M acetic acid) 1% agarose gel (5 g agarose (low electroendosmosis, GOLDBIO, Catalog number: A–201–500) in 500 mL 1X TAE buffer, microwave until transparent) 1 kb DNA ladder (NEB, Catalog number: N3232S) Gel loading dye, purple (6X), no SDS (NEB, Catalog number: B7025S) with SYBR ® Gold nucleic acid gel stain 10,000X concentrate in DMSO (Invitrogen, Catalog number: S11494) Monarch ® PCR & DNA Gel Extraction Kit (NEB) Gibson Mastermix 28 (320 μL 5X isothermal buffer (25% PEG-8000, 500 mM Tris-HCl pH 7.5, 50 mM MgCl 2 , 50 mM dithiothreitol, 1 mM each of the dNTPs, and 5 mM NAD)), 0.64 μL of 10 U/μL T5 exonuclease, 20 μL of 2 U/μL Phusion ® High-Fidelity DNA Polymerase, 160 μL of 40 U/μL Taq DNA ligase from NEB) Purified double-stranded DNA fragments with 18–22 bp overlaps SOC medium (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose, autoclaved) Electrocompetent E. coli strain E. cloni BL21(DE3) cells (Lucigen) LB kan agar plate (15 g agar (molecular genetics grade, Fisher BioReagents TM , Catalog number: BP1423–500) in Luria-Bertani medium with 50 μg/mL kanamycin monosulfate salt, Chem-Impex Int’l. ..



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